Name :
Recombinant Human FGF-10 Protein, HPLC-verified
Biological Activity :
Background :
Fibroblast growth factor 10 (FGF10) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF10 exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. FGF10 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. FGF10 is required for normal branching morphogenesis. It may play a role in wound healing. Defects in FGF10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands (ALSG). ALSG has variable expressivity, and affected individuals may have aplasia or hypoplasia of the lacrimal, parotid, submandibular and sublingual glands and absence of the lacrimal puncta. The disorder is characterized by irritable eyes, recurrent eye infections, epiphora (constant tearing) and xerostomia (dryness of the mouth), which increases the risk of dental erosion, dental caries, periodontal disease and oral infections.
Biological Activity :
1.Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human FGFR2b. The EC50 for this effect is typically 3-30 ng/mL.2.Human lung cancer organoids were cultured with FGF2(Cat#10014-HNAE), FGF4(Cat#16043-HNAE), FGF7(Cat#10210-H07E), EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.3.Human lung organoids were cultured with FGF2(Cat#10014-HNAE), FGF4(Cat#16043-HNAE), FGF7(Cat#10210-H07E), EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.4.Human cholangiocarcinomas organoids were cultured with FGF2(Cat#10014-HNAE), HGF(Cat#10463-HNAS), FGF7(Cat#10210-H07E), EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.5.Human liver cancer organoids were cultured with FGF2(Cat#10014-HNAE), HGF(Cat#10463-HNAS), FGF7(Cat#10210-H07E), EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), TGFB1(Cat#10804-HNAC), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.6.Human kidney cancer organoids were cultured with FGF2(Cat#10014-HNAE), FGF7(Cat#10210-H07E), EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.7.Human kidney organoids were cultured with FGF7(Cat#10210-H07E), EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS), HGF(Cat#10463-HNAS), FGF4(Cat#16043-HNAE). (Routinely tested). Data provided by D1 Medical Technology.8.Human gastric cancer organoids were cultured with EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.9.Human stomach organoids organoids were cultured with EGF(Cat#50482-MNCH), FGF10(Cat#10573-HNAE), NOG(Cat#50688-M02H), RSPO1(Cat#11083-HNAS). (Routinely tested). Data provided by D1 Medical Technology.10.Human small intestinal organoids were cultured with IL22(13059-HNAE), FGF10(10573-HNAE), EGF(50482-MNCH), NOG(50688-M02H). (Routinely tested). Data provided by D1 Medical Technology.
Expression Host :
Human
Source :
E. coli
Tag :
Protein Accession No. :
NP_004456.1
NCBI Gene ID :
Synonyms :
Synonyms :
fibroblast growth factor 10
Amino Acid Sequence :
Molecular Weight :
The recombinant human FGF10 consists of 172 amino acids and has a predicted molecular mass of 19.46 kDa as estimated in SDS-PAGE under reducing conditions.
Purity :
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 5 EU per mg of the protein.
Protein Construction :
A DNA sequence encoding the mature form of human FGF10 (NP_004456.1) (Gln38-Ser208) was expressed with an N-terminal Met.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
KGF2 Protein, Human FGF-10 背景信息 Fibroblast growth factor 10 (FGF10) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF10 exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. FGF10 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. FGF10 is required for normal branching morphogenesis. It may play a role in wound healing. Defects in FGF10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands (ALSG). ALSG has variable expressivity, and affected individuals may have aplasia or hypoplasia of the lacrimal, parotid, submandibular and sublingual glands and absence of the lacrimal puncta. The disorder is characterized by irritable eyes, recurrent eye infections, epiphora (constant tearing) and xerostomia (dryness of the mouth), which increases the risk of dental erosion, dental caries, periodontal disease and oral infections.
References & Citations :
Sekine K, et al. (1999) Fgf10 is essential for limb and lung formation. Nat Genet. 21(1): 138-41.Ohuchi H, et al. (2000) FGF10 acts as a major ligand for FGF receptor 2 IIIb in mouse multi-organ development. Biochem Biophys Res Commun. 277(3): 643-9.Bellusci S, et al. (1997) Fibroblast growth factor 10 (FGF10) and branching morphogenesis in the embryonic mouse lung. Development. 124(23): 4867-78.
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
NPPB Proteincustom synthesis
CD164 ProteinPurity & Documentation
Popular categories:
DDR Family
Toll Like Receptor 10