Name :
Recombinant Human FCGRT & B2M Heterodimer Protein (His Tag), HPLC-verified
Biological Activity :
Background :
Biological Activity :
1. Measured by its ability to bind human IgG1 (Cat: 10702-HNAC) in a functional ELISA.2.Loaded Human FcRn recombinant protein (Cat. CT009-H08H) on His1K Biosensor, can bind IgG1 (Cat. 10702-HNAC) with an affinity constant of 0.11 µM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested).3.Loaded Human FcRn recombinant protein (Cat. CT009-H08H) on His1k Biosensor, can bind IgG4 Fc (Cat. 13505-HNAH) with an affinity constant of 0.08 µM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested).4.Captured Human FcRn recombinant protein (Cat. CT009-H08H) on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.11 µM as determined in an SPR assay (Biacore T200) (Routinely tested).5.Captured Human FcRn recombinant protein (Cat. CT009-H08H) on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.23 µM as determined in an SPR assay (Biacore T200) (Routinely tested).6.The purity of Human FCGRT & B2M Heterodimer Protein (Cat.CT009-H08H) is more than 90% and the molecular weight of this protein is around 45-53 kDa verified by SEC-MALS.7.Loaded Recombinant Human FCGRT & B2M Heterodimer Protein, His Tag (Cat. No. CT009-H08H) on His1K Biosensor, can bind Anti-Erbb2 Antibody, human IgG1 with an affinity constant of 11.1nM as determined in BLI assay (Sartorius Octet RED384) (QC tested).
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
P55899-1 & P61769
NCBI Gene ID :
Synonyms :
Synonyms :
Fc fragment of IgG, receptor, transporter, alpha
Amino Acid Sequence :
Molecular Weight :
The recombinant heterodimer of human FCGRT/B2M comprises 384 (285+99) amino acids and has a calculated molecular mass of 43.5 (31.8+11.7) kDa. The apparent molecular mass of human FCGRT/B2M heterodimer is approximately 35 & 12 kDa respectively in SDS-PAGE under reducing conditions.
Purity :
> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the extracellular domain (Met1-Ser297) of human FCGRT (P55899-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (P61769) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
alpha-chain Protein, Human; FcRn Protein, Human FCGRT & B2M Heterodimer 背景信息
References & Citations :
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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