Name :
Recombinant Human Factor IX Protein (His Tag), HPLC-verified
Biological Activity :
Background :
Coagulation factor IX, also known as Christmas factor, Plasma thromboplastin component and PTC, is a secreted protein which belongs to the peptidase S1 family. Coagulation factor IX / F9 contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one?peptidase S1 domain. Coagulation factor IX / F9 is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ons, phospholipids, and factor VIIIa. Defects in Coagulation factor IX / F9 are the cause of thrombophilia due to factor IX defect which is a hemostatic disorder characterized by a tendency to thrombosis. Defects in Coagulation factor IX / F9 are also the cause of recessive X-linked hemophilia B ( HEMB ) which also known as Christmas disease.
Biological Activity :
Measured by its ability to cleave the peptide substrate, Z-D-Arg-Gly-Arg-pNA. The specific activity is >20pmols/min/ug.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
AAB59620.1
NCBI Gene ID :
Synonyms :
Synonyms :
coagulation factor IX
Amino Acid Sequence :
Molecular Weight :
The secreted recombinant human F9 (pro form) consists of 444 amino acids and predictes a molecular mass of 50 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh F9 is approximately 60-80 kDa due to glycosylation.
Purity :
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human F9 (AAB59620.1) (Met 1-Thr 461) was expressed, fused with a polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
Coagulation factor 9 Protein, Human; Coagulation factor IX Protein, Human; FIX Protein, Human; HEMB Protein, Human; P19 Protein, Human; PTC Protein, Human; THPH8 Protein, Human Factor IX 背景信息 Coagulation factor IX, also known as Christmas factor, Plasma thromboplastin component and PTC, is a secreted protein which belongs to the peptidase S1 family. Coagulation factor IX / F9 contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one?peptidase S1 domain. Coagulation factor IX / F9 is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ons, phospholipids, and factor VIIIa. Defects in Coagulation factor IX / F9 are the cause of thrombophilia due to factor IX defect which is a hemostatic disorder characterized by a tendency to thrombosis. Defects in Coagulation factor IX / F9 are also the cause of recessive X-linked hemophilia B ( HEMB ) which also known as Christmas disease.
References & Citations :
Onay U.V., et al., 2003, Br. J. Haematol. 120:656-659. Vidal F., et al., 2000, Br. J. Haematol. 111:549-551. Simioni P., et al., 2009, N. Engl. J. Med. 361:1671-1675. Espinos C., et al., 2009, Haematologica 88:235-236.
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