Name :
Recombinant Human Factor D Protein (His Tag)
Biological Activity :
Background :
Complement Factor D, also known as Adipsin, C3 convertase activator, Properdin Factor D and CFD is a secreted protein which belongs to thepeptidase S1 family. CFD / Adipsin contains onepeptidase S1 domain. Complement Factor D ( CFD / Adipsin ) is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. Complement Factor D ( CFD / Adipsin ) has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Complement Factor D ( CFD / Adipsin ) cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Complement Factor D ( CFD / Adipsin ) is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Defects in CFD / Adipsin are the cause of complement Factor D deficiency (CFD deficiency) which predisposes to invasive meningococcal disease.
Biological Activity :
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-_x005f_x0002_Lys-ThioBenzyl ester (ZLysSBzl), in the presence of 5,5’Dithiobis (2nitrobenzoic acid) (DTNB).The specific activity is > 70 pmoles/min/μg.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
P00746
NCBI Gene ID :
Synonyms :
Synonyms :
complement Factor D (adipsin)
Amino Acid Sequence :
Molecular Weight :
The secreted recombinant human CFD consists of 239 amino acids and predicts a molecular mass of 25.8 kDa. As a result of glycosylation, rh CFD migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions.
Purity :
> 97 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the mature form of human CFD (P00746) (Ile 26-Ala 253) was fused with a C-terminal polyhistidine tag and a signal peptide at the N-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
ADIPSIN Protein, Human; ADN Protein, Human; DF Protein, Human; Factor D Protein, Human; PFD Protein, Human Factor D 背景信息 Complement Factor D, also known as Adipsin, C3 convertase activator, Properdin Factor D and CFD is a secreted protein which belongs to thepeptidase S1 family. CFD / Adipsin contains onepeptidase S1 domain. Complement Factor D ( CFD / Adipsin ) is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. Complement Factor D ( CFD / Adipsin ) has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Complement Factor D ( CFD / Adipsin ) cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Complement Factor D ( CFD / Adipsin ) is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. Defects in CFD / Adipsin are the cause of complement Factor D deficiency (CFD deficiency) which predisposes to invasive meningococcal disease.
References & Citations :
Volanakis JE, et al.,1996, Protein Sci. 5 (4): 553-64. Searfoss,G.H et al., 2003, J Biol Chem. 278 (46):46107-16. Ukkola,O. et al., 2003, Eur J Clin Nutr. 57 (9):1073-8. Ronti T, et al., 2006, Clin. Endocrinol. (Oxf) 64 (4): 355-65.
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