Name :
Recombinant Human EBP1 Protein (His Tag)
Biological Activity :
Background :
EBP1, also known as PA2G4, is an RNA-binding protein that belongs to the peptidase M24 family. It can be detected n several cell lines tested, including primary and transformed cell lines. EBP1 also present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein is a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. PA2G4 can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. EBP1 has been implicated in growth inhibition and the induction of differentiation of human cancer cells. It seems to be involved in growth regulation. EBP1 also mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site).
Biological Activity :
Testing in progress
Expression Host :
Human
Source :
E. coli
Tag :
Protein Accession No. :
Q9UQ80
NCBI Gene ID :
Synonyms :
Synonyms :
proliferation-associated 2G4
Amino Acid Sequence :
Molecular Weight :
The recombinant human PA2G4 consisting of 404 amino acids and has a calculated molecular mass of 45.2 kDa. It migrates as an approximately 32 KDa band in SDS-PAGE under reducing conditions.
Purity :
> 92 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
Please contact us for more information.
Protein Construction :
A DNA sequence encoding the human PA2G4 (Q9UQ80) (Ser 2-Asp 394) was expressed, with a polyhistide tag at the N-terminus.
Buffer Solution :
Lyophilized from sterile 20mM Tris, 0.5M NaCl, pH 8.0Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
EBP1 Protein, Human; HG4-1 Protein, Human; p38-2G4 Protein, Human EBP1 背景信息 EBP1, also known as PA2G4, is an RNA-binding protein that belongs to the peptidase M24 family. It can be detected n several cell lines tested, including primary and transformed cell lines. EBP1 also present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein is a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. PA2G4 can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. EBP1 has been implicated in growth inhibition and the induction of differentiation of human cancer cells. It seems to be involved in growth regulation. EBP1 also mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site).
References & Citations :
Yoo J Y, et al. (2000) Interaction of the PA2G4 (EBP1) protein with ErbB-3 and regulation of this binding by heregulin. Br J Cancer. 82(3):683-90.Andersen JS, et al. (2002) Directed proteomic analysis of the human nucleolus. Curr Biol. 12(1):1-11.Lehner B, et al. (2004) A protein interaction framework for human mRNA degradation. Genome Res. 14 (7):1315-23.
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