Name :
Recombinant Human DCBLD1 Protein (His Tag)

Biological Activity :

Background :
Discoidin, also known as DCBLD1, contains 1 CUB domain, 1 F5/8 type C domain and 1 LCCL domain. It is a 715 amino acid single-pass membrane protein. Discoidin has a discoidin I-like domain which shares a common C-terminal region of about 110 amino acids with the FA58C domain, but whose N-terminal 40 amino acids are much less conserved. Discoidin gene contains 31 distinct gt-ag introns. Transcription produces 18 different mRNAs, 16 alternatively spliced variants and 2 unspliced forms. Discoidin is expected to localize in various compartments.

Biological Activity :
Testing in progress

Expression Host :
Human

Source :
HEK293 Cells

Tag :

Protein Accession No. :
Q8N8Z6-2

NCBI Gene ID :

Synonyms :

Synonyms :
discoidin, CUB and LCCL domain containing 1

Amino Acid Sequence :

Molecular Weight :
The recombinant human DCBLD1 consists of 436 amino acids and predicts a molecular mass of 48.4 KDa. It migrates as an approximately 65 KDa band in SDS-PAGE under reducing conditions.

Purity :
> 95 % as determined by SDS-PAGE

State of Matter :

Product Concentration :

Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method

Protein Construction :
A DNA sequence encoding the human DCBLD1 (Q8N8Z6-2) (Met1-Val459) was expressed with a polyhistidine tag at the C-terminus.

Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.

Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Synonyms :
dJ94G16.1 Protein, Human; RP1-94G16.1 Protein, Human DCBLD1 背景信息 Discoidin, also known as DCBLD1, contains 1 CUB domain, 1 F5/8 type C domain and 1 LCCL domain. It is a 715 amino acid single-pass membrane protein. Discoidin has a discoidin I-like domain which shares a common C-terminal region of about 110 amino acids with the FA58C domain, but whose N-terminal 40 amino acids are much less conserved. Discoidin gene contains 31 distinct gt-ag introns. Transcription produces 18 different mRNAs, 16 alternatively spliced variants and 2 unspliced forms. Discoidin is expected to localize in various compartments.

References & Citations :
The MGC Project Team. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 14:2121-27.Horton R, et al. (2003) The DNA sequence and analysis of human chromosome 6. Nature 425:805-11.Suzuki Y, et al. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36:40-5.

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