Name :
Recombinant Human CTLA-4 Protein (His Tag), HPLC-verified
Biological Activity :
Background :
Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg). Cancer Immunotherapy Co-inhibitory Immune Checkpoint Targets CTLA4 / CD152 Immune Checkpoint Proteins Immune Checkpoint Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: WB Antibodies Immune Checkpoint Proteins Immune Checkpoint Targets Immunotherapy Targeted Therapy
Biological Activity :
1. Immobilized Recombinant Human CD86 / B7-2 Protein (Fc Tag)(Cat:10699-H02H) at 2 μg/ml (100 μl/well) can bind Recombinant Human CTLA-4 Protein (His Tag) (Cat:11159-H08H), the EC50 is 2.6-8 ng/mL.2. Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.05-0.3μg/mL when stimulated with 1 μg/mL Recombinant Human B7‑1/CD80.3.Captured Ipilimumab (68052-H001) on proA Chip can bind CTLA-4 (Cat.No.11159-H08H) with an affinity constant of 20.03nM as determined in a SPR assay (Biacore T200)(Routinely tested).4.Loaded Ipilimumab on ProA Biosensor, can bind CTLA-4 Protein, Human, Recombinant, His Tag(Cat.No.11159-H08H) with an affinity constant of 5.15nM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested).5.Loaded Recombinant Human B7-1 Protein, hFc Tag (Cat. No. 10698-H02H) on ProA Biosensor, can bind Recombinant Human CTLA-4 Protein, His Tag (Cat. No. 11159-H08H) with an affinity constant of 2.66nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).6.Loaded Recombinant Human CD86 Protein, hFc Tag (Cat. No. 10699-H02H) on ProA Biosensor, can bind Recombinant Human CTLA-4 Protein, His Tag (Cat. No. 11159-H08H) with an affinity constant of 5.34nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_005205.2
NCBI Gene ID :
Synonyms :
Synonyms :
cytotoxic T-lymphocyte-associated protein 4
Amino Acid Sequence :
Molecular Weight :
The recombinant human CTLA4 consists of 138 amino acids and has a predicted molecular mass of 15 kDa. As a result of glycosylation, the apparent molecular mass of human CTLA4 is approximately 22-27 kDa in SDS-PAGE under reducing conditions
Purity :
> 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human CTLA4 (NP005205.2) extracellular domain(Lys36-Phe162) was fused with the a polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
ALPS5 Protein, Human; CD Protein, Human; CD152 Protein, Human; CELIAC3 Protein, Human; CTLA-4 Protein, Human; GRD4 Protein, Human; GSE Protein, Human; IDDM12 Protein, Human CTLA-4 背景信息 Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg). Cancer Immunotherapy Co-inhibitory Immune Checkpoint Targets CTLA4 / CD152 Immune Checkpoint Proteins Immune Checkpoint Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: WB Antibodies Immune Checkpoint Proteins Immune Checkpoint Targets Immunotherapy Targeted Therapy
References & Citations :
Slavik JM, et al. (1999) CD28/CTLA-4 and CD80/CD86 families: signaling and function. Immunol Res. 19(1): 1-24.Holmberg D, et al. (2005) CTLA-4 (CD152) and its involvement in autoimmune disease. Autoimmunity. 38(3): 225-33.Chin LT, et al. (2008) Immune intervention with monoclonal antibodies targeting CD152 (CTLA-4) for autoimmune and malignant diseases. Chang Gung Med J. 31(1): 1-15.
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