Name :
Recombinant Human CLEC4C Protein (ECD, hFc Tag)
Biological Activity :
Background :
C-type Lectin Domain Family 4 Member C (CLEC4C) is a transmembrane protein selectively expressed on plasmocytoid dendritic cells (pDCs) which regulates secretion of proinflammatory cytokines and type I interferons in pDCs exposed to TLR7 and 9 agonists such as microbial CpG DNA. Its gene located on chromosome 12p13.31, CLEC4C consists of a 21 amino acid (aa) N- cytoplasmic domain, a 23 aa transmembrane segment, and a 169 aa extracellular domain that contains a juxtamembrane neck region and a C-carbohydrate recognition domain. Blastic plasmacytoid dendritic cell neoplasm is positive specifically for CLEC4C. Monoclonal antibodies directed against CLEC4C have achieved disease control in patients with cutaneous lupus erythematosus. CLEC4C-mediated suppression of IFNα production is regulated by the masking/unmasking of galactose moieties, representing a new potential strategy for regulating immune responses in pathological conditions.
Biological Activity :
Testing in progress
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NCBI Gene ID :
Synonyms :
Synonyms :
Amino Acid Sequence :
Molecular Weight :
The recombinant Human CLEC4C consisting of 401 amino acids and has a calculated molecular mass of 46.02 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 53.1 kDa protein in SDS-PAGE under reducing conditions.
Purity :
≥ 95 % as determined by SDS-PAGE.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the Human CLEC4C (NP_569708.1) (Asn45-Ile213) was expressed with the Fc region of human IgG1 at the N-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
BDCA2 Protein, Human; CD303 Protein, Human; CHC Protein, Human; CHC17 Protein, Human; CLECSF11 Protein, Human; CLECSF7 Protein, Human; CLH-17 Protein, Human; CLTCL2 Protein, Human; DLEC Protein, Human; Hc Protein, Human; HECL Protein, Human; MRD56 Protein, Human; PRO34150 Protein, Human; UNQ9361/PRO34150 Protein, Human CLEC4C 背景信息 C-type Lectin Domain Family 4 Member C (CLEC4C) is a transmembrane protein selectively expressed on plasmocytoid dendritic cells (pDCs) which regulates secretion of proinflammatory cytokines and type I interferons in pDCs exposed to TLR7 and 9 agonists such as microbial CpG DNA. Its gene located on chromosome 12p13.31, CLEC4C consists of a 21 amino acid (aa) N- cytoplasmic domain, a 23 aa transmembrane segment, and a 169 aa extracellular domain that contains a juxtamembrane neck region and a C-carbohydrate recognition domain. Blastic plasmacytoid dendritic cell neoplasm is positive specifically for CLEC4C. Monoclonal antibodies directed against CLEC4C have achieved disease control in patients with cutaneous lupus erythematosus. CLEC4C-mediated suppression of IFNα production is regulated by the masking/unmasking of galactose moieties, representing a new potential strategy for regulating immune responses in pathological conditions.
References & Citations :
1. Wu P, et al. TLR9/TLR7-triggered downregulation of BDCA2 expression on human plasmacytoid dendritic cells from healthy individuals and lupus patients. Clin Immunol. 2008, doi: 10.1016/j.clim.2008.06.0042. Lim SM, et al. CLEC4C p.K210del variant causes impaired cell surface transport in plasmacytoid dendritic cells of amyotrophic lateral sclerosis. Oncotarget. 2016, doi: 10.18632/oncotarget.78863. Robak E, Braun M, Robak T. Leukemia Cutis-The Current View on Pathogenesis, Diagnosis, and Treatment. Cancers (Basel). 2023, doi: 10.3390/cancers152253934. Wilson NR, et al. CD303 (BDCA-2) – a potential novel target for therapy in hematologic malignancies. Leuk Lymphoma. 2022, doi: 10.1080/10428194.2021.19751925. Riboldi E, et al. Human C-type lectin domain family 4, member C (CLEC4C/BDCA-2/CD303) is a receptor for asialo-galactosyl-oligosaccharides. J Biol Chem. 2011, doi: 10.1074/jbc.C111.290494
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