Name :
Recombinant Human Claudin-9 Protein (His & AVI Tag), Biotinylated, Nanodisc
Biological Activity :
Background :
Claudin-9 (CLDN9) belongs to the claudins family and is a transmembrane protein found in tight junctions with two extracellular loops and a cytoplasmic C tail. CLDN9 modulates the ion- and charge-specific permeability of the paracellular pathway in most epithelial tissues. It forms heterotypic interactions with other claudins to create cation-selective channels in the kidney and may contribute to the maintenance of alveolar barrier function in the lung. Deficiency is shown to be associated with autosomal recessive deafness, DFNB116. CLDN9 expression has been shown to be upregulated in certain types of cancer, such as endometrial cancer and hepatocellular carcinoma, and its knockdown has been found to reduce cell proliferation and migration in vitro.
Biological Activity :
1.Immobilized Human CLDN9-Nanodisc Protein(His & AVI Tag), Biotinylated (Cat:21185-H49H-NA-B）at 5 μg/mL (100 μL/well) on streptavidin precoated (2 μg/mL，100 μL/well) can bind Anti-Human Claudin9 Antibody, Human IgG1,the EC50 is 10-60 ng/mL.2.Loaded Biotinylated Recombinant Human CLDN9-Nanodisc Protein, His & AVI Tag(Cat:21185-H49H-NA-B) on SA Biosensor, can bind Anti-CLDN9 antibody with an affinity constant of 11.15 nM as determined in BLI assay (ForteBio Octet Red384) (Routinely tested).
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
O95484
NCBI Gene ID :
Synonyms :
Synonyms :
Amino Acid Sequence :
Molecular Weight :
The recombinant human CLDN9 consists of 209 amino acids and has a predicted molecular mass of 22.44 kDa.
Purity :
>80% as determined by SDS-PAGE.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -70℃. Store it under sterile conditions at -70℃ or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the human CLDN9 (O95484) (Met1-Thr184) was expressed, with a polyhistidine tag followed by an AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins’ nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
Buffer Solution :
Supplied as 0.2 μm filtered solution in 10 mM HEPES, 50 mM NaCl, pH7.5 with glycerol as protectant.Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
It is supplied and shipped as liquid with dry ice.
Redissolution :
Synonyms :
References & Citations :
1. Higashi, et al. Claudin-9 constitutes tight junctions of folliculo-stellate cells in the anterior pituitary gland. Sci Rep, 2021. 2. Nakano, et al. A claudin-9-based ion permeability barrier is essential for hearing. PLoS Genet., 2009. Zheng A, et al. Claudin-6 and claudin-9 function as additional coreceptors for hepatitis C virus. J Virol. 2007.3. Endo Y, et al. Claudin 9 is a novel prognostic biomarker for endometrial cancer. Int J Oncol. 2022.4. Overgaard, C. E., Mitchell, L. A., & Koval, M. Roles for claudins in alveolar epithelial barrier function. Annals of the New York Academy of Sciences, 2012.5. Endo, et al. Claudin 9 is a novel prognostic biomarker for endometrial cancer. International Journal of Oncology, 2022.6. Liu, et al. Claudin-9 enhances the metastatic potential of hepatocytes via Tyk2/Stat3 signaling. The Turkish Journal of Gastroenterology, 2019.
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