Name :
Recombinant Human Claudin18 Protein (Nanodisc, Full Length, His & Strep Tag)
Biological Activity :
Background :
Claudins family member Claudin18 (CLDN18) is a four-pass transmembrane protein with two extracellular loops and cytoplasmic N- and C-tails found in tight junctions. CLDN18 modulates paracellular permeability, polarity, and signaling. CLDN18 deficiency has been associated with atrophic gastritis, spasmolytic polypeptide-expressing metaplasia (SPEM), and asthma. Lung-specific CLDN18.1 modulates alveolar epithelial type II (AT2) cell proliferation and organ size. Restoration of CLDN18.1 expression in human lung cancer cells has been shown to suppress proliferation, inhibiting the IGF-1R/AKT axes. Not expressed in other healthy tissues, gastric mucosa-specific isoform CLDN18.2 expression manifests in primary gastric cancers. CLDN18.2 at the surface of epithelial tumor cells is a target in antibody and CAR-therapies currently in development.
Biological Activity :
1.Loaded Anti-Human Claudin-18.2 antibody, human IgG1 on ProA Biosensor, can bind Recombinant Human Claudin-18.2-Nanodisc (Full Length) Protein, His & Strep Tag(Cat. No. 30025-H64H-NA) with an affinity constant of 3.43 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).2.Immobilized Recombinant Human CLDN18.2 -Nanodisc (Full Length) Protein(His & Strep Tag)(Cat:30025-H64H-NA) at 5 μg/mL (100 μL/well) can bind Anti-Human claudin18.2 Antibody, Human IgG1, the EC50 is 4-12 ng/mL.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
P56856-2
NCBI Gene ID :
Synonyms :
Synonyms :
claudin 18
Amino Acid Sequence :
Molecular Weight :
The recombinant Human CLDN18.2-Nanodisc consists of 303 amino acids and predicts a molecular mass of 32.34 kDa. It migrates as an approximately 27.5 kDa band in SDS-PAGE under reducing conditions.
Purity :
≥ 75 % as determined by SDS-PAGE.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -70℃. Store it under sterile conditions at -70℃ or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
Please contact us for more information.
Protein Construction :
A DNA sequence encoding the Human CLDN18.2-Nanodisc (Full Length) (P56856-2) (Met1-Val261) was expressed, with a polyhistidine tag at the N-terminus and a Strep tag at the C-terminus.Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins’ nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
Buffer Solution :
Supplied as sterile 10 mM Hepes, 50mM NaCl, 1% glycerol, pH 7.5.Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
It is supplied and shipped as liquid with dry ice.
Redissolution :
Synonyms :
SFTA5 Protein, Human; SFTPJ Protein, Human Claudin18 背景信息 Claudins family member Claudin18 (CLDN18) is a four-pass transmembrane protein with two extracellular loops and cytoplasmic N- and C-tails found in tight junctions. CLDN18 modulates paracellular permeability, polarity, and signaling. CLDN18 deficiency has been associated with atrophic gastritis, spasmolytic polypeptide-expressing metaplasia (SPEM), and asthma. Lung-specific CLDN18.1 modulates alveolar epithelial type II (AT2) cell proliferation and organ size. Restoration of CLDN18.1 expression in human lung cancer cells has been shown to suppress proliferation, inhibiting the IGF-1R/AKT axes. Not expressed in other healthy tissues, gastric mucosa-specific isoform CLDN18.2 expression manifests in primary gastric cancers. CLDN18.2 at the surface of epithelial tumor cells is a target in antibody and CAR-therapies currently in development.
References & Citations :
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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