Name :
Recombinant Human Claudin-6 Protein (His & FLAG Tag), Nanodisc
Biological Activity :
Background :
Claudin-6 (CLDN6) belongs to the claudins family and is a transmembrane protein found in tight junctions with two extracellular loops and intracellular N and C tails. CLDN6 modulates the ion- and charge-specific permeability of the paracellular pathway in most epithelial tissues. It regulates the paracellular flux of cations in the kidney through forming heterotypic strands with other claudins and has a role in maintaining the lung epithelial barrier function. The CLDN6 gene is adjacent to another family member, CLDN9, on chromosome 16. Certain CLDN6-positive cancers, encompassing ovarian, endometrial, and testicular malignancies, have been shown to have their proliferation and invasiveness suppressed upon CLDN6 silencing.
Biological Activity :
1.Loaded Anti-CLDN6 antibody on ProA Biosensor, can bind Human CLDN6 Protein(Cat：16658-H61H-NA) with an affinity constant of 0.536nM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested).2.Captured Anti-Human CLDN6 antibody on CM5 Chip via anti-human IgG Fc antibody, can bind Recombinant Human CLDN6-Nanodisc Protein (His & Flag Tag) (Cat：16658-H61H-NA) with an affinity constant of 7.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
P56747
NCBI Gene ID :
Synonyms :
Synonyms :
claudin 6
Amino Acid Sequence :
Molecular Weight :
The recombinant human CLDN6 consists of 202 amino acids and has a predicted molecular mass of 21.87 kDa. The apparent molecular mass of it is approximately 20.91 KDa in SDS-PAGE under reducing conditions.
Purity :
≥ 90 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to three months from date of receipt at -70℃. Store it under sterile conditions at -70℃ or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the human CLDN6 (P56747) (Met1-Thr184) was expressed, with a Flag tag followed by a polyhistidine tag at the C-terminus.Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins’ nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
Buffer Solution :
Supplied as 0.2 μm filtered solution in 10 mM HEPES, 50 mM NaCl, pH7.5 with glycerol as protectant.Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
It is supplied and shipped as liquid with dry ice.
Redissolution :
Synonyms :
References & Citations :
1. Qu H, Jin Q, Quan C. CLDN6: From Traditional Barrier Function to Emerging Roles in Cancers. Int J Mol Sci. 2021.2. Du H, et al. Claudin 6: Therapeutic prospects for tumours, and mechanisms of expression and regulation (Review). Mol Med Rep. 2021.3. Brad Screnci, et. al. Atomic-level specificity of Claudin 6 monoclonal antibodies isolated for treating solid tumors [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022.4. Huang L, et al. Downregulation of CLDN6 inhibits cell proliferation, migration, and invasion via regulating EGFR/AKT/mTOR signalling pathway in hepatocellular carcinoma. Cell Biochem Funct. 2020.
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