Name :
Recombinant Human Creatine Kinase Muscle/CKM Protein (His Tag)
Biological Activity :
Background :
CKM, also known as CK-MM, is mainly expressed in skeletal muscle. As the primary CK isoenzyme, it also can be detected in heart muscle. CKM is a subunit of creatine kinase (CK). CK is an enzyme expressed by various tissues and cell types. It catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). In the cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type).
Biological Activity :
Kinase activity untested
Expression Host :
Human
Source :
E. coli
Tag :
Protein Accession No. :
AAH07462.1
NCBI Gene ID :
Synonyms :
Synonyms :
creatine kinase, muscle
Amino Acid Sequence :
Molecular Weight :
The recombinant human CKM consists of 396 amino acids and predicts a molecular mass of 44.9 KDa. It migrates as an approximately 45 KDa band in SDS-PAGE under reducing conditions.
Purity :
> 90 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
Please contact us for more information.
Protein Construction :
A DNA sequence encoding the human CKM (AAH07462.1) (Met1-Lys381) was expressed with a polyhistide tag at the N-terminus.
Buffer Solution :
Supplied as sterile 100mM HEPES, pH 7.0.Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Redissolution :
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Synonyms :
CKMM Protein, Human; M-CK Protein, Human Creatine Kinase Muscle/CKM 背景信息 CKM, also known as CK-MM, is mainly expressed in skeletal muscle. As the primary CK isoenzyme, it also can be detected in heart muscle. CKM is a subunit of creatine kinase (CK). CK is an enzyme expressed by various tissues and cell types. It catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). In the cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type).
References & Citations :
Goldblatt H. 1969, Laboratory Investigation. 21 (2): 126-8.Hekimsoy Zeliha. et al., 2005, Endocrine Research. 31 (3): 171-5.Döring F. et al., 2011, Scand J Med Sci Sports. 21(6):841-5.
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