Name :
Recombinant Human CD3e & CD3G Heterodimer Protein (Fc-Flag & Fc-His Tag), HPLC-verified

Biological Activity :

Background :

Biological Activity :
Immobilized Anti-CD3 antibody, mouse IgG2a at 2 μg/mL (100 μL/well) can bind Recombinant Human CD3e & CD3G Heterodimer Protein(Fc – FLAG & Fc – His Tag) (Cat: CT123-H2303H), the EC50 is 50-150 ng/mL.

Expression Host :
Human

Source :
HEK293 Cells

Tag :

Protein Accession No. :
NP_000724.1 & NP_000064.1

NCBI Gene ID :

Synonyms :

Synonyms :
CD3e molecule, epsilon (CD3-TCR complex)

Amino Acid Sequence :

Molecular Weight :
The recombinant Human CD3e & CD3G consists of 694 (353+341) amino acids and has a calculated molecular mass of 78.36 (39.77+38.59) KDa.As a result of glycosylation, the apparent molecular mass of mouse CD3e & CD3G heterodimer is approximately 47.10 and 42.93 KDa in SDS-PAGE under reducing conditions.

Purity :
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

State of Matter :

Product Concentration :

Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.

Protein Construction :
A DNA sequence encoding the (Met1-Asp126) of human CD3e (NP_000724.1) was fused with a C-terminal Fc region of human IgG1 tag followed by a FLAG tag, constructed the plasmid 1; A DNA sequence encoding the (Met1-Ser116) of human CD3G (NP_000064.1) was fused with a C-terminal Fc region of human IgG1 tag followed by a polyhistidine tag, constructed the plasmid 2. The two plasmids were co-expressed and the Human CD3e/CD3G heterodimer was purified.

Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.

Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Synonyms :
CD3e & CD3G Heterodimer Protein, Human; IMD18 Protein, Human; T3E Protein, Human; TCRE Protein, Human CD3e & CD3G Heterodimer 背景信息

References & Citations :

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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