Name :
Recombinant Human CD32B/Fcgr2b Protein (ECD, His Tag), HPLC-verified
Biological Activity :
Background :
Biological Activity :
1.Immobilized Human FcγRIIB/C / CD32b/c recombinant protein (Cat. 10259-H08C) can bind biotinylated human IgG1, The EC50 is 60-180 ng/mL.2.Loaded Human FcγRIIB/C / CD32b/c recombinant protein (Cat. 10259-H08C) on His1K Biosensor, can bind Rituximab (IgG1) with an affinity constant of 1.5 μM as determined in a BLI assay (Sartorius Octet Red384).3.Captured IgG1 on proteinA Chip can bind Human FcγRIIB/C / CD32b/c recombinant protein (Cat. 10259-H08C) with an affinity constant of 2.9 μM as determined in an SPR assay (Biacore T200).4.Captured Human CD32b/c recombinant protein（10259-H08C）on Anti-His Chip can bind IgG1 Fc Protein, His (Cat. 10702-HNAC) with an affinity constant of 37.5 μM as determined in an SPR assay (Biacore T200).
Expression Host :
Human
Source :
CHO Cells
Tag :
Protein Accession No. :
NP_001002274.1
NCBI Gene ID :
Synonyms :
Synonyms :
Fc fragment of IgG, low affinity IIb, receptor (CD32)
Amino Acid Sequence :
Molecular Weight :
The secreted recombinant human CD32b consists of 183 amino acids and predicts a molecular mass of 20.8 KDa. The apparent molecular mass of the protein is approximately 22-29 KDa in SDS-PAGE under reducing conditions due to different glycosylation.
Purity :
> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding (Ala46-Pro217) of human CD32b (NP_001002274.1) was expressed with a C-terminal polyhistidine tag.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
CD32 Protein, Human; CD32A Protein, Human; CD32B Protein, Human; CDw32 Protein, Human; Fc gamma RII Protein, Human; Fc gamma RIIB Protein, Human; FCG2 Protein, Human; FcGR Protein, Human; FCGR2 Protein, Human; FCGR21 Protein, Human; FCGR2A Protein, Human; IGFR2 Protein, Human CD32B/Fcgr2b 背景信息
References & Citations :
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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