Name :
Recombinant Human CD32A/FCGR2A Protein (His & AVI Tag), Biotinylated, HPLC-verified
Biological Activity :
Background :
Biological Activity :
1.Loaded Biotinylated Human FcγRIIA / CD32a (H167) recombinant protein (His & Avi Tag) (Cat. 10374-H27H1-B) on SA Biosensor, can bind Rituximab (IgG1) with an affinity constant of 0.53 μM as determined in a BLI assay (Sartorius Octet Red384) (QC tested).2.The purity of Human CD32a / FCGR2A Protein (167 His, His & AVI Tag), Biotinylated (Cat.10374-H27H1-B) is more than 95% and the molecular weight of this protein is around 20-38 kDa verified by SEC-MALS.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
P12318-1
NCBI Gene ID :
Synonyms :
Synonyms :
Fc fragment of IgG receptor IIa
Amino Acid Sequence :
Molecular Weight :
The recombinant human CD32a consists of 213 amino acids after removal of the signal peptide and predicts a molecular mass of 23.7 KDa. It migrates as an approximately 33 KDa band due to glycosylation by SDS-PAGE under reducing conditions.
Purity :
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the extracellular domain (Met1-Met210) of human CD32a (P12318-1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
CD32 Protein, Human; CD32A Protein, Human; CDw32 Protein, Human; Fc gamma RIIA Protein, Human; FCG2 Protein, Human; FcGR Protein, Human; FCGR2 Protein, Human; FCGR2A1 Protein, Human; IGFR2 Protein, Human CD32A/FCGR2A 背景信息
References & Citations :
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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