Name :
Recombinant Human CD32A/FCGR2A Protein (167 Arg, hFc Tag)

Biological Activity :

Background :

Biological Activity :
Measured by its binding ability in a functional ELISA.Immobilized human CD32a-Fc (Cat:10374-H02H) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1 with a linear range of 0.625-10 μg/ml.

Expression Host :
Human

Source :
HEK293 Cells

Tag :

Protein Accession No. :
AAA35827.1

NCBI Gene ID :

Synonyms :

Synonyms :
Fc fragment of IgG receptor IIa

Amino Acid Sequence :

Molecular Weight :
The recombinant human CD32a/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 426 amino acids and predicts a molecular mass of 47.3 kDa. As a result of glycosylation, the apparent molecular mass of rh CD32a/Fc monomer is approximately 55 kDa in SDS-PAGE under reducing conditions.

Purity :
> 90 % as determined by SDS-PAGE

State of Matter :

Product Concentration :

Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method

Protein Construction :
A DNA sequence encoding the extracellular domain (Met 1-Ile 218) of human CD32a (AAA35827.1) was fused with the Fc region of human IgG1 at the C-terminus. It is identical to FCGR2A131H/R in the reference.

Buffer Solution :
Lyophilized from sterile PBS, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.

Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Synonyms :
CD32 Protein, Human; CD32A Protein, Human; CDw32 Protein, Human; Fc gamma RIIA Protein, Human; FCG2 Protein, Human; FcGR Protein, Human; FCGR2 Protein, Human; FCGR2A1 Protein, Human; IGFR2 Protein, Human CD32A/FCGR2A 背景信息

References & Citations :

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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