Name :
Recombinant Human CD1D & B2M Heterodimer Protein (His Tag), HPLC-verified

Biological Activity :

Background :

Biological Activity :
Immobilized anti-CD1D (1D12), Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human CD1D & B2M Heterodimer Protein (Cat: CT042-H08H), the EC50 is 70-210 ng/mL.

Expression Host :
Human

Source :
HEK293 Cells

Tag :

Protein Accession No. :
P15813 & P61769

NCBI Gene ID :

Synonyms :

Synonyms :

Amino Acid Sequence :

Molecular Weight :
The recombinant heterodimer of human CD1D/B2M comprises 393 (293+100) amino acids and has a calculated molecular mass of 45 (33.3+ 11.7) KDa. The apparent molecular mass of rh CD1D/B2M heterodimer is approximately 43.2 and 12.5 KDa respectively in SDS-PAGE under reducing conditions.

Purity :
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

State of Matter :

Product Concentration :

Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method

Protein Construction :
A DNA sequence encoding the extracellular domain (Met1-Ser301) of human CD1D (P15813) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (P61769) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the CD1D/B2M heterodimer was purified.

Buffer Solution :
Lyophilized from sterile 25mM Sodium Citrate, 200mM Nacl, 0.02% Tween 80, pH 6.0.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.

Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Synonyms :

References & Citations :

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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