Name :
Recombinant Human CD16b/FCGR3B/Fc gamma RIIIB Protein (NA1 allotype, His & AVI Tag), Biotinylated, HPLC-verified
Biological Activity :
Background :
Biological Activity :
1.Loaded Biotinylated Human FcγRIIIB / CD16b (NA1) recombinant protein (His & Avi Tag) (Cat. 11046-H27H1-B) on SA Biosensor, can bind Rituximab (IgG1) with an affinity constant of 4.3 μM as determined in BLI assay (Sartorius Octet Red384)(QC tested).2.The purity of CD16b Protein, Human, Recombinant (NA1 allotype, His & AVI Tag) (Cat.11046-H27H1-B) is more than 90% and the molecular weight of this protein is around 26-44 kDa verified by SEC-MALS.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
AAA35881.1
NCBI Gene ID :
Synonyms :
Synonyms :
Fc fragment of IgG receptor IIIb
Amino Acid Sequence :
Molecular Weight :
The recombinant human FCGR3B consists of 207 amino acids and predicts a molecular mass of 23.75 kDa. As a result of glycosylation, it migrates as an approximately 44.02 kDa band in SDS-PAGE under reducing conditions.
Purity :
> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the human FCGR3B (AAA35881.1) (Met1-Ser200) was expressed with a c-terminal AVI tagged polyhistidine tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
CD16 Protein, Human; CD16b Protein, Human; Fc gamma RIIIb Protein, Human; FCG3 Protein, Human; FCGR3 Protein, Human; FCR-10 Protein, Human; FCRIII Protein, Human; FCRIIIb Protein, Human CD16b/FCGR3B/Fc gamma RIIIB 背景信息
References & Citations :
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
EphA7 Protein
ENPP3 Protein
Popular categories:
KIR2DS3
CD200R4