Name :
Recombinant Human CD16b/FCGR3B/Fc gamma RIIIB Protein (NA2 allotype, His Tag), HPLC-verified
Biological Activity :
Background :
Biological Activity :
1.Loaded Human FcγRIIIB / CD16b (NA2) recombinant protein (Cat. 11046-H08H) on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 8.3 μM as determined in a BLI assay (Sartorius Octet Red384) (QC tested).2.Captured Human FcγRIIIB / CD16b (NA2) recombinant protein (Cat. 11046-H08H) on Anti-His Chip can bind IgG1 with an affinity constant of 2.6 μM as determined in an SPR assay (Biacore T200) (Routinely tested).3.Captured IgG1 on proteinA Chip can bind Human FcγRIIIB / CD16b (NA2) recombinant protein (Cat. 11046-H08H) with an affinity constant of 0.22 μM as determined in an SPR assay (Biacore T200) (Routinely tested).4.The purity of Human CD16b / FCGR3B Protein (His Tag, NA2 allotype) (Cat.11046-H08H) is more than 95% and the molecular weight of this protein is around 33-43 kDa verified by SEC-MALS.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
O75015.2
NCBI Gene ID :
Synonyms :
Synonyms :
Fc fragment of IgG receptor IIIb
Amino Acid Sequence :
Molecular Weight :
The recombinant human CD16b comprises 195 amino acids with a predicted molecular mass of 22.2 kDa. As a result of glycosylation, rh CD16b migrates as an approximately 46 kDa band in SDS-PAGE under reducing conditions.
Purity :
> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human CD16b (O75015.2) (Met 1-Ser 200) was fused with a polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
CD16 Protein, Human; CD16b Protein, Human; Fc gamma RIIIb Protein, Human; FCG3 Protein, Human; FCGR3 Protein, Human; FCR-10 Protein, Human; FCRIII Protein, Human; FCRIIIb Protein, Human CD16b/FCGR3B/Fc gamma RIIIB 背景信息
References & Citations :
MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
IL-5 Protein
CD117/c-Kit Protein
Popular categories:
CG-alpha
Carboxypeptidase Q