Name :
Recombinant Human CD16a/FCGR3A/Fc gamma RIIIA Protein (ECD, 176F, His & AVI Tag), Biotinylated, HPLC-verified
Biological Activity :
Background :
The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells.CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.
Biological Activity :
1.Loaded Biotinylated Human FcγRIIIA / CD16a (F176) recombinant protein (His & Avi Tag) (Cat. 10389-H27H-B) on SA Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.26 μM as determined in a BLI assay (Sartorius Octet Red384) (QC tested).2.Loaded Rituximab (IgG1) on ProA Biosensor, can bind Biotinylated Human FcγRIIIA / CD16a (F176) recombinant protein (His & Avi Tag) (Cat. 10389-H27H-B) with an affinity constant of 0.23 μM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested).3.The purity of Human CD16a / FCGR3A Protein (176 Phe, His & AVI Tag), Biotinylated (Cat.10389-H27H-B) is more than 95% and the molecular weight of this protein is around 31-47 kDa verified by SEC-MALS.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
P08637-1
NCBI Gene ID :
Synonyms :
Synonyms :
Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Amino Acid Sequence :
Molecular Weight :
The secreted recombinant human CD16a consists of 223 amino acids and predicts a molecular mass of 25.1 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 48 kDa due to glycosylation.
Purity :
> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the extracellular domain (Met1-Gln208) of human CD16a (P08637-1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus (AVI-his). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
CD16 Protein, Human; CD16A Protein, Human; Fc gamma RIIIa Protein, Human; FCG3 Protein, Human; FCGR3 Protein, Human; FCGRIII Protein, Human; FCR-10 Protein, Human; FCRIII Protein, Human; FCRIIIA Protein, Human; IGFR3 Protein, Human; IMD20 Protein, Human CD16a/FCGR3A/Fc gamma RIIIA 背景信息 The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells.CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.
References & Citations :
David Dornan, et al. Effect of FCGR2A and FCGR3A variants on CLL outcome. Blood. 2010 116: 4212-4222
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