Name :
Human CCR2-Nanodisc Protein (Isoform B, His & Flag Tag)
Biological Activity :
Background :
The C-C motif chemokine receptor-2 (CCR2) is a G protein-coupled receptor (GPCR), made up of a carboxy-terminus, extracellular amino terminus, and a hydrophobic transmembrane domain consisting of 7 amino acid segments that mediates agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. CCR2 is expressed on monocytes, immature dendritic cells, and T-cell subpopulations, and mediates their migration towards endogenous CCL2. CCR2 is necessary for macrophage-dependent inflammatory responses and the development of atherosclerosis. In mice, CCR2 deficiency reduced macrophage content, increased adiponectin expression, ameliorated hepatic steatosis, and improved systemic glucose homeostasis and insulin sensitivity. Resistance to HIV-1 infection or delayed progression to AIDS may be linked to CCR2 polymorphisms. Furthermore, CCR2 mRNA was highly expressed in prostate cancer (PCa) metastatic tissues compared with benign prostate tissues according to real-time RT-PCR3, suggesting that CCR2 may contribute to PCa development.
Biological Activity :
1.Immobilized recombinant Human CCR2b Protein (Cat: 16084-H18H-NA) at 5 μg/mL (100 μL/well) can bind anti-CCR2 Antibody, the EC50 is 9-27 ng/mL.2.Loaded anti-CCR2 Antibody, Human IgG1 on ProA Biosensor, can bind Human CCR2-Nanodisc Protein, His & Flag Tag (Cat. No. 16084-H18H-NA) with an affinity constant of 29.9 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
P41597-2
NCBI Gene ID :
Synonyms :
Synonyms :
chemokine (C-C motif) receptor 2
Amino Acid Sequence :
Molecular Weight :
The recombinant human CCR2b predicts a molecular mass of 49.12 kDa. The apparent molecular mass of the protein is approximately 41 kDa in SDS-PAGE under reducing conditions.
Purity :
≥ 90 % as determined by SDS-PAGE.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to three months from date of receipt at -70℃. Store it under sterile conditions at -70℃ or lower. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the human CCR2b (P41597-2) (Leu2-Arg231 & Glu235-Leu360, thermostabilizing mutations) (TM domains 1-7) was expressed and linked by a Fusion Protein, a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus. Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins’ nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
Buffer Solution :
Supplied as sterile 10mM Hepes, 50mM NaCl, pH 7.5.Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
It is supplied and shipped as liquid with dry ice.
Redissolution :
Synonyms :
CC-CKR-2 Protein, Human; CCR-2 Protein, Human; CCR2A Protein, Human; CCR2B Protein, Human; CD192 Protein, Human; CKR2 Protein, Human; CKR2A Protein, Human; CKR2B Protein, Human; CMKBR2 Protein, Human; MCP-1-R Protein, Human CCR2 背景信息 The C-C motif chemokine receptor-2 (CCR2) is a G protein-coupled receptor (GPCR), made up of a carboxy-terminus, extracellular amino terminus, and a hydrophobic transmembrane domain consisting of 7 amino acid segments that mediates agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. CCR2 is expressed on monocytes, immature dendritic cells, and T-cell subpopulations, and mediates their migration towards endogenous CCL2. CCR2 is necessary for macrophage-dependent inflammatory responses and the development of atherosclerosis. In mice, CCR2 deficiency reduced macrophage content, increased adiponectin expression, ameliorated hepatic steatosis, and improved systemic glucose homeostasis and insulin sensitivity. Resistance to HIV-1 infection or delayed progression to AIDS may be linked to CCR2 polymorphisms. Furthermore, CCR2 mRNA was highly expressed in prostate cancer (PCa) metastatic tissues compared with benign prostate tissues according to real-time RT-PCR3, suggesting that CCR2 may contribute to PCa development.
References & Citations :
1. Zheng, Y., et al. Structure of CC chemokine receptor 2 with orthosteric and allosteric antagonists. Nature, 2016. doi: 10.1038/nature206052. Weisberg SP, et al. CCR2 modulates inflammatory and metabolic effects of high-fat feeding. J Clin Invest. 2006. doi: 10.1172/JCI243353. Lu Y, et al. CCR2 expression correlates with prostate cancer progression. J Cell Biochem. 2007. doi: 10.1002/jcb.21220
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