Name :
Recombinant Human Casein Kinase 1 alpha Protein (GST Tag)
Biological Activity :
Background :
Casein kinase I isoform alpha, also known as CKI-alpha, CK1 and CSNK1A1, is a cytoplasm protein which belongs to theprotein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. High expression of CSNK2A1, or concomitantly high expression of CSNK2A1, are independent prognostic factors of poor survival in NSCLC patients. CSNK2A1 are useful prognosis markers in non-small cell lung cancer (NSCLC) patients after complete resection, independent of lymph node metastasis status. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. It phosphorylates CTNNB1 at ‘Ser-45’. CSNK1A1 may play a role in segregating chromosomes during mitosis.
Biological Activity :
The specific activity was determined to be 30 nmol/min/mg using casein as substrate.
Expression Host :
Human
Source :
Baculovirus-Insect Cells
Tag :
Protein Accession No. :
P48729-1
NCBI Gene ID :
Synonyms :
Synonyms :
casein kinase 1, alpha 1
Amino Acid Sequence :
Molecular Weight :
The recombinant human CSNK1A1/GST chimera consists of 561 amino acids and predicts a molecular mass of 65.2 kDa. It migrates as an approximately 62 kDa band in SDS-PAGE under reducing conditions.
Purity :
> 85 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the full length of human CSNK1A1 isoform 1 (P48729-1) (Met 1-Phe 337) was expressed with the GST tag at the N-terminus.
Buffer Solution :
Supplied as sterile 20mM Tris, 500mM NaCl, 2mM GSH, pH 7.4Please contact us for any concerns or special requirements.Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Redissolution :
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Synonyms :
CK1 Protein, Human; CK1a Protein, Human; CKIa Protein, Human; HEL-S-77p Protein, Human; HLCDGP1 Protein, Human; PRO2975 Protein, Human Casein Kinase 1 alpha 背景信息 Casein kinase I isoform alpha, also known as CKI-alpha, CK1 and CSNK1A1, is a cytoplasm protein which belongs to theprotein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. High expression of CSNK2A1, or concomitantly high expression of CSNK2A1, are independent prognostic factors of poor survival in NSCLC patients. CSNK2A1 are useful prognosis markers in non-small cell lung cancer (NSCLC) patients after complete resection, independent of lymph node metastasis status. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. It phosphorylates CTNNB1 at ‘Ser-45’. CSNK1A1 may play a role in segregating chromosomes during mitosis.
References & Citations :
Dubois, et al.,2002, FEBS Lett. (Netherlands) 517 (1-3): 167-71. Dubois, T et al.,2001, J. Biol. Chem.(United States) 276 (22): 18757-64. Zhang, Yi et al., 2002, J. Biol. Chem. 277(20): 17706-12. Wang,Z. et al., 2010, Med Sci Monit.16 (8):CR357-64.
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