Name :
Recombinant Human Cadherin-11 Protein (His Tag), HPLC-verified
Biological Activity :
Background :
A mesenchymal marker, Cadherin-11‘s (CDH11) gene is situated on chromosome 16q22.1. CDH11 is a type II atypical cadherin, an integral membrane protein that mediates calcium-dependent, cell-cell homophilic interactions. Defined by a large extracellular N-domain, a single membrane-spanning domain, a small C-cytoplasmic domain, and the lack of an HAV cell adhesion recognition sequence, CDH11 expression is increased in fibrotic diseases such as systemic sclerosis, as well as in chronic inflammatory arthritis. CDH11 leads to promoter methylation inactivation, which can induce cancer cell apoptosis and suppress invasion through Wnt/β-catenin, AKT/Rho A, and NF-κB signaling pathways. CDH11 mRNA and protein was expressed in most invasive breast cancer cell lines tested but not in non-invasive cell lines, suggesting that CDH11 may be a marker for aggressive, invasive tumor subsets.
Biological Activity :
Testing in progress
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NCBI Gene ID :
Synonyms :
Synonyms :
cadherin 11, type 2, OB-cadherin (osteoblast)
Amino Acid Sequence :
Molecular Weight :
The recombinant Human CDH11 consisting of 575 amino acids and has a calculated molecular mass of 63.38 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 67.5 kDa protein in SDS-PAGE under reducing conditions.
Purity :
≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the Human CDH11 (NP_001788) (Gly54-Thr617) was expressed with a polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile 50mM Tris, 100mM NaCl, 3mM Cacl2, 10% Trehalose, 0.02% Tween20, pH 8.0.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
CAD11 Protein, Human; CDHOB Protein, Human; OB Protein, Human; OSF-4 Protein, Human Cadherin-11 背景信息 A mesenchymal marker, Cadherin-11‘s (CDH11) gene is situated on chromosome 16q22.1. CDH11 is a type II atypical cadherin, an integral membrane protein that mediates calcium-dependent, cell-cell homophilic interactions. Defined by a large extracellular N-domain, a single membrane-spanning domain, a small C-cytoplasmic domain, and the lack of an HAV cell adhesion recognition sequence, CDH11 expression is increased in fibrotic diseases such as systemic sclerosis, as well as in chronic inflammatory arthritis. CDH11 leads to promoter methylation inactivation, which can induce cancer cell apoptosis and suppress invasion through Wnt/β-catenin, AKT/Rho A, and NF-κB signaling pathways. CDH11 mRNA and protein was expressed in most invasive breast cancer cell lines tested but not in non-invasive cell lines, suggesting that CDH11 may be a marker for aggressive, invasive tumor subsets.
References & Citations :
1.Chen X, et al. Research progress in the role and mechanism of Cadherin-11 in different diseases. J Cancer. 2021 Jan 1;12(4):1190-1199. doi: 10.7150/jca.52720. PMID: 33442417.2.Sfikakis, Petros P. et al. Cadherin-11 as a therapeutic target in chronic, inflammatory rheumatic diseases. Clinical Immunology, 2017. doi:10.1016/j.clim.2017.01.008 3.Thandiwe Chavula, Sarah To, Sandeep K. Agarwal. Cadherin-11 and Its Role in Tissue Fibrosis. Cells Tissues Organs, 2023. https://doi.org/10.1159/0005253594.Satriyo PB, et al. Cadherin 11 Inhibition Downregulates β-catenin, Deactivates the Canonical WNT Signalling Pathway and Suppresses the Cancer Stem Cell-Like Phenotype of Triple Negative Breast Cancer. J Clin Med. 2019 Jan 27;8(2):148. doi: 10.3390/jcm8020148. PMID: 30691241.
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