Name :
Recombinant Human CA5A Protein (His Tag)
Biological Activity :
Background :
Carbonic Anhydrase 5A, mitochondrial, also known as Carbonate dehydratase VA, Carbonic Anhydrase VA, CA-VA and CA5A, is a member of thealpha-Carbonic Anhydrase family. Carbonic Anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA5A / CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt).
Biological Activity :
Measured by its esterase activity. The specific activity is >500 pmoles/min/μg.
Expression Host :
Human
Source :
E. coli
Tag :
Protein Accession No. :
NP_001730.1
NCBI Gene ID :
Synonyms :
Synonyms :
Carbonic Anhydrase VA, mitochondrial
Amino Acid Sequence :
Molecular Weight :
The recombinant human CA5A consisting of 277 amino acids and has a calculated molecular mass of 31.6 kDa. It migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions.
Purity :
> 96 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
Please contact us for more information.
Protein Construction :
A DNA sequence encoding the mature form of human CA5A (NP_001730.1) (Ala 40-Ser 305) was fused with an Met at N-terminus and a polyhistide tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile 50mM NaAc, 50mM NaCl, 0.05% Brij 35, pH 5.0Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
CA5 Protein, Human; CA5AD Protein, Human; CA5D Protein, Human; Carbonic Anhydrase VA Protein, Human; CAV Protein, Human; CAVA Protein, Human; GS1-21A4.1 Protein, Human CA5A 背景信息 Carbonic Anhydrase 5A, mitochondrial, also known as Carbonate dehydratase VA, Carbonic Anhydrase VA, CA-VA and CA5A, is a member of thealpha-Carbonic Anhydrase family. Carbonic Anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA5A / CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt).
References & Citations :
Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899 – 903. Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 – 262. Temperini C.et al., 2006, Chemistry 12: 7057-66. Temperini C.et al., 2006, J. Med. Chem. 49: 3019-27. Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602. Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222.
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