Name :
Recombinant Human B7-1/CD80 Protein (ECD, His Tag), HPLC-verified
Biological Activity :
Background :
The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas. Cancer Immunotherapy Co-inhibitory Immune Checkpoint Targets Immune Checkpoint Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: IHC Antibodies Immune Checkpoint Detection: WB Antibodies Immune Checkpoint Proteins Immune Checkpoint Targets Immunotherapy Targeted Therapy
Biological Activity :
1.Measured by its binding ability in a functional ELISA. Immobilized B7-1 Protein, Human, Recombinant (His Tag) (Cat: 10698-H08H) at 2 μg/ml (100 μl/well) can bind CTLA-4 Protein, Human, Recombinant (His & hFc Tag) (Cat: 11159-H03H). The EC50 of of CTLA-4 (His & hFc Tag) (Cat: 11159-H03H) is 35-80 ng/ml.2.Loaded Recombinant Human CTLA-4 Protein, hFc Tag (Cat.No.11159-H02H6) on ProA Biosensor, can bind Recombinant Human B7-1 Protein, His Tag (Cat.No.10698-H08H) with an affinity constant of 5.331nM as determined in BLI assay (Sartorius Octet RH16) (Routinely tested).3.Loaded Recombinant Human B7-1 Protein, His Tag(Cat. No. 10698-H08H) on His1K Biosensor, can bind Recombinant Human CD28 Protein, hFc Tag (Cat. No. 11524-H02H) with an affinity constant of 0.46 µM as determined in BLI assay (Sartorius Octet RED384) (QC tested).4.Loaded Recombinant Human CD86 Protein, His Tag(Cat. No. 10699-H08H) on His1K Biosensor, can bind Recombinant Human CD28 Protein, hFc Tag (Cat. No. 11524-H02H) with an affinity constant of 2.72 µM as determined in BLI assay (Sartorius Octet RED384) (QC tested).
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_005182.1
NCBI Gene ID :
Synonyms :
Synonyms :
CD80 molecule
Amino Acid Sequence :
Molecular Weight :
The recombinant human B7-1 consists of 219 amino acids and has a predicted molecular mass of 25.4 kDa. By reduced SDS-PAGE, the apparent molecular mass of rh B7-1 is approximately 45-48 kDa due to glycosylation.
Purity :
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the extracellular domain (Met 1-Asn 242) of human B7-1 (NP_005182.1) precursor was fused with a polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
B7 Protein, Human; B7-1 Protein, Human; B7.1 Protein, Human; BB1 Protein, Human; CD28LG Protein, Human; CD28LG1 Protein, Human; LAB7 Protein, Human B7-1/CD80 背景信息 The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas. Cancer Immunotherapy Co-inhibitory Immune Checkpoint Targets Immune Checkpoint Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: IHC Antibodies Immune Checkpoint Detection: WB Antibodies Immune Checkpoint Proteins Immune Checkpoint Targets Immunotherapy Targeted Therapy
References & Citations :
Greenfield EA, et al. (1998) CD28/B7 costimulation: a review. Crit Rev Immunol. 18(5): 389-418.Zang X, et al. (2007) The B7 family and cancer therapy: costimulation and coinhibition. Clin Cancer Res. 13(18 Pt 1): 5271-9.Mir MA, et al. (2008) Signaling through CD80: an approach for treating lymphomas. Expert Opin Ther Targets. 12(8): 969-79.
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