Name :
Recombinant Human Alkaline Phosphatase/ALPL Protein (His Tag)
Biological Activity :
Background :
Alkaline Phosphatase (ALPL) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, Alkaline Phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Alkaline Phosphatases (APs) are ubiquitous in many species, from bacteria to human. Four genes encode AP isoenzymes in humans and rodents. Three AP genes are expressed in a tissue-specific manner (i.e., placental, embryonic, and intestinal AP isoenzymes). Expression of the fourth AP gene is nonspecific to a single tissue and is especially abundant in bone, liver, and kidney. This isoenzyme is also called tissue-nonspecific Alkaline Phosphatase (TNAP). The enzyme tissue non-specific Alkaline Phosphatase (TNAP) belongs to the ectophosphatase family. TNAP is present in large amounts in bone in which it plays a role in mineralization.
Biological Activity :
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP).The specific activity is > 50, 000 pmoles/min/μg.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_000469.3
NCBI Gene ID :
Synonyms :
Synonyms :
Alkaline Phosphatase, liver/bone/kidney
Amino Acid Sequence :
Molecular Weight :
The recombinant human ALPL consists of 496 amino acids and predicts a molecular mass of 55 kDa. As a result of glycosylation, rhALPL migrates as approximately 65 kDa band in SDS-PAGE under reducing conditions.
Purity :
≥ 95 % as determined by SDS-PAGE.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human tissue-nonspecific Alkaline Phosphatase (NP_000469.3) (Met 1-Ser 502) was expressed with a C-terminal polyhistidine tag.
Buffer Solution :
Lyophilized from 20mM HEPES, 150mM NaCl, 2mM MgSO4, 0.1mM ZnCl2,pH 7.5.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
AP-TNAP Protein, Human; APTNAP Protein, Human; HOPS Protein, Human; TNAP Protein, Human; TNSALP Protein, Human Alkaline Phosphatase/ALPL 背景信息 Alkaline Phosphatase (ALPL) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, Alkaline Phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Alkaline Phosphatases (APs) are ubiquitous in many species, from bacteria to human. Four genes encode AP isoenzymes in humans and rodents. Three AP genes are expressed in a tissue-specific manner (i.e., placental, embryonic, and intestinal AP isoenzymes). Expression of the fourth AP gene is nonspecific to a single tissue and is especially abundant in bone, liver, and kidney. This isoenzyme is also called tissue-nonspecific Alkaline Phosphatase (TNAP). The enzyme tissue non-specific Alkaline Phosphatase (TNAP) belongs to the ectophosphatase family. TNAP is present in large amounts in bone in which it plays a role in mineralization.
References & Citations :
Brun-Heath I, et al. (2011) Differential expression of the bone and the liver tissue non-specific Alkaline Phosphatase isoforms in brain tissues. Cell Tissue Res. 343(3): 521-36.Whyte MP, et al. (1995) Alkaline Phosphatase: placental and tissue-non-specific isoenzymes hydrolyze phosphoethanolamine, inorganic pyrophosphate, and pyridoxal 5-phosphate. J Clin Invest. 95: 1440-5.Whyte MP. (1994) Hypophosphatasia and the role of Alkaline Phosphatase in skeletal mineralization. Endocrinol Rev. 4: 439-61.Weinreb M, et al. (1990) Different pattern of Alkaline Phosphatase, osteopontin and osteocalcin expression in developing rat bone visualized by in situ hybridization. J Bone Miner Res. 5: 831-42.
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