Name :
Recombinant Human Alkaline phosphatase/APLI Protein (hFc Tag)
Biological Activity :
Background :
ALPI encodes for intestinal phosphatase alkaline, a brush border metalloenzyme that hydrolyses phosphate from the lipid A moiety of lipopolysaccharides and thereby drastically reduces Toll-like receptor 4 agonist activity. ALPI mutations impaired either stability or catalytic activity of ALPI and rendered it unable to detoxify lipopolysaccharide-dependent signalling. ALPI mutations should be included in screening for monogenic causes of inflammatory bowel diseases and lay the groundwork for ALPI-based treatments in intestinal inflammatory disorders.
Biological Activity :
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP).The specific activity is > 10,000 pmoles/min/μg.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
P09923
NCBI Gene ID :
Synonyms :
Synonyms :
alkaline phosphatase, intestinal
Amino Acid Sequence :
Molecular Weight :
The secreted recombinant human ALPI/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 725 amino acids and has a predicted molecular mass of 79.5 kDa. The apparent molecular mass of rhALPI /Fc monomer is approximately 90-95 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity :
> 83 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the mature form of human ALPI (P09923) (Met 1-Asp 503),without the pro peptide, was expressed with the fused Fc region of human IgG1 at the C-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
IAP Protein, Human Alkaline phosphatase/APLI 背景信息 ALPI encodes for intestinal phosphatase alkaline, a brush border metalloenzyme that hydrolyses phosphate from the lipid A moiety of lipopolysaccharides and thereby drastically reduces Toll-like receptor 4 agonist activity. ALPI mutations impaired either stability or catalytic activity of ALPI and rendered it unable to detoxify lipopolysaccharide-dependent signalling. ALPI mutations should be included in screening for monogenic causes of inflammatory bowel diseases and lay the groundwork for ALPI-based treatments in intestinal inflammatory disorders.
References & Citations :
Nakano T, et al. (2009) Role of lysophosphatidylcholine in brush-border intestinal alkaline phosphatase release and restoration. Am J Physiol Gastrointest Liver Physiol. 297(1): G207-14.Henthorn PS, et al. (1987) Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: close homology to placental alkaline phosphatase. Proc Natl Acad Sci. 84(5): 1234-8.Oetting WS, et al. (2003) Oculocutaneous albinism type 1: the last 100 years. Pigment Cell Res. 16(3): 307-11.
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