Name :
Recombinant Aequorea victoria GFP Protein (His Tag), HPLC-verified
Biological Activity :
Background :
The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSparkTM is mainly intended for applications where fast appearance of bright fluorescence is crucial. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. It is specially recommended for cell and organelle labeling and tracking the promoter activity.
Biological Activity :
Activity Assay
Expression Host :
Aequorea victoria
Source :
E. coli
Tag :
Protein Accession No. :
AAB65663
NCBI Gene ID :
Synonyms :
Synonyms :
Amino Acid Sequence :
Molecular Weight :
The recombinant Aequorea victoria GFP consisting of 253 amino acids and has a calculated molecular mass of 28.7 kDa. It migrates as an approximately 34 kDa band in SDS-PAGE under reducing conditions as predicted.
Purity :
≥ 90 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
Please contact us for more information.
Protein Construction :
A DNA sequence encoding the Aequorea victoria GFP (AAB65663) (Ser2-Lys238) was expressed with a polyhistide tag at the N-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH7.4Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
GFP Protein, Aequorea victoria GFP 背景信息 The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSparkTM is mainly intended for applications where fast appearance of bright fluorescence is crucial. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. It is specially recommended for cell and organelle labeling and tracking the promoter activity.
References & Citations :
Evdokimov et al. (2006). EMBO Rep, 7 (10):1006–1012 / pmid: 16936637. Haas et al. (1996). Curr Biol, 6 (3): 315–324 / pmid: 8805248.Kremers et al. (2006). Biochemistry, 45 (21): 6570–6580 / pmid: 16716067. Li et al. (1998). J Biol Chem, 273 (52): 34970–34975 /pmid: 9857028. Reid and Flynn (1997). Biochemistry, 36 (22): 6786–6791 / pmid: 9184161.Shagin et al. (2004). Curr Biol, 21 (5): 841–850 / pmid: 14963095.
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