Name :
Recombinant Human Prostatic Acid Phosphatase/ACPP Protein (His Tag), HPLC-verified
Biological Activity :
Background :
Prostatic Acid Phosphatase (PAP, or ACPP), also known as prostatic specific Acid Phosphatase (PSAP), is an enzyme produced by the prostate. As a non-specific phosphomonoesterase, Prostatic Acid Phosphatase is synthesized and secreted into seminal plasma under androgenic control. The enzyme is a dimer of molecular weight around 100 kDa. Prostatic Acid Phosphatase is a clinically important protein for its relevance as a biomarker of prostate carcinoma. Furthermore, it has a potential role in fertilization. The major action of PAP is to dephosphorylate macromolecules with the help of catalytic residues (His(12) and Asp(258)) that are located in the cleft between two domains. Cellular prostatic Acid Phosphatase (cPAcP), an authentic tyrosine phosphatase, is proposed to function as a negative growth regulator of prostate cancer (PCa) cells in part through its dephosphorylation of ErbB-2. cPAcP functions as a neutral protein tyrosine phosphatase (PTP) in prostate cancer cells and dephosphorylates HER-2/ErbB-2/Neu (HER-2: human epidermal growth factor receptor-2) at the phosphotyrosine (p-Tyr) residues. Injection of the secretory isoform of PAP has potent antinociceptive effects in mouse models of chronic pain. This enzyme exhibits ecto-5′-nucleotidase activity, is widely distributed, and implicated in the formation of chronic pain. Additionally, PAP could be a target molecule in specific immunotherapy for patients with nonprostate adenocarcinomas including colon and gastric cancers.
Biological Activity :
Measured by its ability to cleave a substrate, pNitrophenyl phosphate (pNPP). The specific activity is >100,000 pmol/min/μg.
Expression Host :
Human
Source :
HEK293 Cells
Tag :
Protein Accession No. :
NP_001127666.1
NCBI Gene ID :
Synonyms :
Synonyms :
Acid Phosphatase, prostate
Amino Acid Sequence :
Molecular Weight :
The recombinant human ACPP consists of 361 amino acids and has a predicted molecular mass of 42 kDa. The apparent molecular mass of rh ACPP is approximately 45-55 kDa in SDS-PAGE under reducing conditions.
Purity :
≥ 97 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human ACPP (NP_001127666.1) (Met 1-Lys 382) was fused with a polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
5′-NT Protein, Human; ACP-3 Protein, Human; ACP3 Protein, Human; PAP Protein, Human Prostatic Acid Phosphatase/ACPP 背景信息 Prostatic Acid Phosphatase (PAP, or ACPP), also known as prostatic specific Acid Phosphatase (PSAP), is an enzyme produced by the prostate. As a non-specific phosphomonoesterase, Prostatic Acid Phosphatase is synthesized and secreted into seminal plasma under androgenic control. The enzyme is a dimer of molecular weight around 100 kDa. Prostatic Acid Phosphatase is a clinically important protein for its relevance as a biomarker of prostate carcinoma. Furthermore, it has a potential role in fertilization. The major action of PAP is to dephosphorylate macromolecules with the help of catalytic residues (His(12) and Asp(258)) that are located in the cleft between two domains. Cellular prostatic Acid Phosphatase (cPAcP), an authentic tyrosine phosphatase, is proposed to function as a negative growth regulator of prostate cancer (PCa) cells in part through its dephosphorylation of ErbB-2. cPAcP functions as a neutral protein tyrosine phosphatase (PTP) in prostate cancer cells and dephosphorylates HER-2/ErbB-2/Neu (HER-2: human epidermal growth factor receptor-2) at the phosphotyrosine (p-Tyr) residues. Injection of the secretory isoform of PAP has potent antinociceptive effects in mouse models of chronic pain. This enzyme exhibits ecto-5′-nucleotidase activity, is widely distributed, and implicated in the formation of chronic pain. Additionally, PAP could be a target molecule in specific immunotherapy for patients with nonprostate adenocarcinomas including colon and gastric cancers.
References & Citations :
Hassan MI, et al. (2010) Structural and functional analysis of human prostatic Acid Phosphatase. Expert Rev Anticancer Ther. 10(7): 1055-68.Chuang TD, et al. (2010) Human prostatic Acid Phosphatase, an authentic tyrosine phosphatase, dephosphorylates ErbB-2 and regulates prostate cancer cell growth. J Biol Chem. 285(31): 23598-606.Larsen RS, et al. (2009) A high throughput assay to identify small molecule modulators of prostatic Acid Phosphatase. Curr Chem Genomics. 3: 42-9.Zimmermann H. (2009) Prostatic Acid Phosphatase, a neglected ectonucleotidase. Purinergic Signal. 5(3): 273-5.Wang Y, et al. (2005) Prostatic Acid Phosphatase as a target molecule in specific immunotherapy for patients with nonprostate adenocarcinoma. J Immunother. 28(6): 535-41.Veeramani S, et al. (2005) Cellular prostatic Acid Phosphatase: a protein tyrosine phosphatase involved in androgen-independent proliferation of prostate cancer. Endocr Relat Cancer. 12(4): 805-22.Ostrowski WS, et al. (1994) Human prostatic Acid Phosphatase: selected properties and practical applications. Clin Chim Acta. 226(2): 121-9.
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