Name :
Recombinant Human 15 Lipoxygenase 2 Protein (His & GST Tag)
Biological Activity :
Background :
ALOX15B is a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. ALOX15B converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. ALOX15B gene is located in a cluster of related genes and a pseudogene that spans approximately 1 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described.
Biological Activity :
Testing in progress
Expression Host :
Human
Source :
Baculovirus-Insect Cells
Tag :
Protein Accession No. :
AAH35217.1
NCBI Gene ID :
Synonyms :
Synonyms :
arachidonate 15-lipoxygenase, type B
Amino Acid Sequence :
Molecular Weight :
The secreted recombinant human ALOX15B consists of 913 amino acids and predicts a molecular mass of 103.7 KDa. The apparent molecular mass of the protein is approximately 93 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity :
> 90 % as determined by SDS-PAGE
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg of the protein as determined by the LAL method
Protein Construction :
A DNA sequence encoding the human ALOX15B (AAH35217.1)( Met1-Ile676) was expressed the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Buffer Solution :
Lyophilized from sterile 20mM Tris, 500mM Nacl, 10% glycerol, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
15-LOX-2 Protein, Human; ALOX15B Protein, Human 15 Lipoxygenase 2 背景信息 ALOX15B is a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. ALOX15B converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. ALOX15B gene is located in a cluster of related genes and a pseudogene that spans approximately 1 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described.
References & Citations :
Kilty I. et al., 2000, Eur J Biochem. 266 (1): 83-93. Sigal E. et al., 1990, J Biol Chem. 265 (9): 5113-20. Brash AR. et al., 1997, Proc Natl Acad Sci. 94 (12): 6148-52.
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