Alterations (log2 values) and probability values (log10) for person murine mRNAs, comparing handle hearts and Allo 3d. ACR, acute cellular rejection; ISHLT, International Society of Heart and Lung Transplantation; mRNA, messenger RNA.American Journal of Transplantation 2016; 16: 99RNA Mechanisms in Acute Allograft RejectionFigure 4: Differentially regulated human and mouse mRNA transcripts from the micro array studies. (A) Pathway purchase IC87201 enrichment evaluation of human mRNA arrays (ISHLT3 vs. manage) showed substantial enrichment of predominantly immune-related pathways (Table S7). The heat map, showing in rows all genes represented within the enriched pathways, confirm that the ISHLT3 cardiac samples are very regulated except for one particular. Furthermore, gene expression in ISHLT0 cardiac samples doesn’t substantially differ from handle gene expression, as becomes apparent in the mixed unsupervised clustering of these groups. (B) Pathway enrichment evaluation of murine mRNA arrays (Allo7d vs. handle) showed substantial enrichment of predominantly metabolic and immune-related pathways (Table S8). The heat maps, displaying in rows all genes represented in these pathways, confirm that the Allo7d samples are very regulated and that the Allo3d samples are inside a transition phase in which the respective genes are mildly regulated. Additionally, the unsupervised clustering of the samples based on the gene expression of these pathways results inside the great discrimination of handle, Allo3d, and Allo7d samples. ISHLT, International Society of Heart and Lung Transplantation; mRNA, messenger RNA.Figure five: ACR in miR-155 and miR-155 mice following HTX. (A) Experimental setup of miR-155 and miR-155 mice in spontaneous survival experiments. (B) Graft failure occurred later when BALB/cJ hearts have been transplanted into miR-155 versus miR155 mice. (C) In spite of longer exposure to the host’s immune system, BALB/cJ grafts weighed significantly less PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/20082338 when transplanted into miR-155 mice. (D) Experimental setup of miR-155 and miR-155 mice sacrificed on a fixed time point (5d) following HTX. (E) Whereas the amount of infiltrating CD45 and Mac3-positive cells was drastically significantly less in BALB/cJ hearts transplanted into miR155 versus miR-155 mice, the number of CD3-positive cells did not significantly differ among genotypes (p 0.05). ACR, acute cellular rejection; MST, median survival time; HTX, heart transplantation; miR, microRNA.American Journal of Transplantation 2016; 16: 99Van Aelst et alFigure 6: ACR in anti-155 and anti-scram injected mice following HTX. (A) Experimental setup of anti-155 and anti-scram injected mice sacrificed on a fixed time point (5d) following HTX. (B) Normalized expression levels of miR-155 in (un) transplanted hearts following anti-155 and anti cram injection. (C) Normalized splenic expression levels of miR-155 in (un) transplanted mice following injection with anti-155 and anti-scram. (D) BALB/cJ grafts weighed significantly much less when transplanted into anti-155 injected mice. The amount of infiltrating CD45 (E) and Mac3-positive (F) cells was drastically less in BALB/cJ hearts transplanted into anti-155 versus anti-scram injected mice. (G) The amount of CD3-positive cells was not substantially various amongst treatment options. (H) Following HTX, splenic SPI1 decreased in anti-scram mice, whereas it remained elevated in anti-155 mice. Splenic SPI1 was significantly larger in transplanted anti-155 versus anti-scram injected mice (p 0.05; p 0.01). ACR, a.