Product Name: cGMP Enzyme Immunoassay Kit, Direct
Product Type: Chemical
CAS NO: 321-55-1c-Fms inhibitors
application(s)
ELISA: suitable
Shipped in: wet ice
Storage temp.: −20°C
usage
sufficient for 96 assays
Application: Non-radioactive, competitive immunoassay for the quantitation of total cGMP in tissue and cell cultures. Buffer contains 0.1N HCl that aids in the lysis of cells, inhibits endogenous phosphodiesterases and stabilizes the cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is perFormed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.
Features and Benefits:
Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perForm in your application, we will issue a full credit or replacement antibody. Learn more.
Symbol
GHS02, GHS05, GHS07
Signal word
Danger
Hazard statements
H226-H302 + H312 + H332-H314-H335
Precautionary statements
P261-P280-P305 + P351 + P338-P310
Hazard Codes
C
Risk Statements
10-20/21/22-35
Safety Statements
26-36/37/39-45
RIDADR: UN 3316 9
WGK Germany: 3
Flash Point(F)
120.2 °F
Flash Point(C)
49 °C
Storage Temp.: −20°C
UNSPSC
12352200
Components
0.1 M Hydrochloride Acid 1 x 30 mL; Acetic Anhydride 1 x 1 mL; cGMP-Alkaline Phosphatase Conjugate 1 x 5 mL; cGMP EIA Antibody Rabbit Anti-cGMP 1 x 5 mL; Cyclic GMP Standard 1 x 0.5 mL; Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea; Neutralizing Reagent 1 x 6 mL; p-Nitrophenyl Phosphate Substrate Solution 1 x 20 mL; Plate Sealer 1 ea; Stop Solution 1 x 5 mL; Triethylamine 1 x 2 mL; Wash Buffer Concentrate 1 x 30 mL
PubMed ID:http://jpet.aspetjournals.org/content/98/1/72