Product Name: Glutathione Peroxidase Cellular Activity Assay Kit
Synonym: Cellular glutathione peroxidase; c-GPx
Product Type: Chemical
CAS NO: 1642-54-2FLT3 inhibitors
shelf life
Kit is stable for 24 months when unopened.
Shipped in: wet ice
Storage temp.: −20°C
usage
sufficient for 100 tests
Frequently Asked Questions:
Frequently Asked Questions are available for this Product.
General description: The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.
Other Notes:
This kit was tested with rabbit reticulocyte lysate and with glutathione peroxidase enzyme.
Symbol
GHS02, GHS05, GHS06, GHS08, GHS09
Signal word
Danger
Hazard statements
H226-H242-H302-H311 + H331-H314-H317-H341-H411
Precautionary statements
P220-P261-P273-P280-P305 + P351 + P338-P310
Hazard Codes
O,T,N
Risk Statements
7-10-21/22-23-34-43-51/53-68
Safety Statements
3/7-17-26-36/37/39-45-61
RIDADR: UN 3316 9
WGK Germany: 3
Storage Temp.: −20°C
UNSPSC
12352200
Components
tert-Butyl hydroperoxide 1 mL; Glutathione Peroxidase Assay Buffer 120 mL; NADPH Assay Reagent 5 vials
PubMed ID:http://jpet.aspetjournals.org/content/98/2/121