Product Name: CelLytic™ MEM Protein Extraction Kit
Product Type: Chemical
CAS NO: 113558-14-8Ack1 inhibitors
Shipped in: wet ice
Storage temp.: −20°C
Application: The kit offers a fast and convenient method to isolate hydrophobic and raft microdomain associated proteins from cells. The method is based on phase separation and does not require cell membrane isolation. The separated proteins can be used for further experiments such as SDS-PAGE, Western blotting, dot blotting, and immunoprecipitation. The kit has been tested on, but not limited to, HeLa, HEK-293, NIH 3T3, COS and CHO cell lines.
General description: Membrane proteins make up around 20-30% of an organism′s genome and serve as cellular gatekeepers, regulators, and sensors. They have diverse cellular functions, such as shielding the cell from external toxins, being the starting point of intracellular signaling cascades, and retaining critical ion concentrations.
Legal InFormation: CelLytic is a trademark of Sigma-Aldrich Co. LLC
Suitability:
Sufficient reagents supplied for 80 tests.
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
Storage Temp.: −20°C
UNSPSC
12352200
Components
Lysis and Separation Buffer 50 mL; Wash Buffer for CelLytic MEM 50 mL; Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 1 mL; Sodium Chloride, 4M Solution 1.5 mL
PubMed ID:http://jpet.aspetjournals.org/content/98/1/45