Toluidine blue staining confirmed cells with standard dim-blue or purple cytoplasmic granules resembling mast cells in the mesenteric window of WT mice
The next working day, the tissues ended up incubated for one hour at place temperature with the secondary antibody donkey anti-rat CY5 conjug1297537-33-7ated (1.1000, Jackson ImmunoResearch). Immunolabeled tissues ended up…