The reverse primer was. The templates for truncated (103 nt fifty nine UTR) YB-1 mRNA, truncated (seventy two nt fifty nine UTR) YB-one mRNA, and truncated (36 nt fifty nine UTR) YB-one mRNA in vitro synthesis had been obtained by PCR with complete HeLa cDNA as template
Gene particular primers for 59RACE had been an substitute variety of YB-one mRNA in complete RNA was detected utilizing PCR with HEK293 and MCF7 whole cDNA CB-5083(or HEK293 and MCF7…