This was assessed by a comet assay, which quantifies double-stranded DNA (dsDNA) breaks, in T98G cells transfected with miR-221 or a scrambled sequence and then taken care of with TMZ at distinct times
Alkaline comet assay was performed appropriately to manufacturer's guidance (Trevigen, Gaithersburg, Maryland, Usa). Briefly, 12x104 glioblastoma mobile lines ended up transfected with miRs or MGMT cDNA and then treated with…